α粒子诱发细胞癌变的纺锤体检测点功能分析

doi:10.3969/j.issn.1004-616x.2003.02.001

癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (2): 65-66.doi: 10.3969/j.issn.1004-616x.2003.02.001

• 论文 • 下一篇

α粒子诱发细胞癌变的纺锤体检测点功能分析

隋建丽，孙敬芬，周平坤，吴德昌

军事医学科学院放射医学研究所，北京　100850

收稿日期:2002-11-07 修回日期:2003-01-07 出版日期:2003-04-30 发布日期:2003-04-30
通讯作者: 周平坤

THE SPINDLE CHECKPOINT MECHANISM OF BEP2D AND ITS MALIGNANT TRANSFORMED CELL LINES INDUCED BY α PARTICLES EXPOSURE

SUI Jian-li, SUN Jing-fen, ZHOU Ping-kun, et al.

Department of Radiation Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China

Received:2002-11-07 Revised:2003-01-07 Online:2003-04-30 Published:2003-04-30
Contact: ZHOU Ping-kun

摘要/Abstract

摘要： 目的：通过分析人支气管上皮细胞BEP2D和其α粒子诱发的癌变细胞系BERP35T1和BERP35T4的纺锤体检测点功能，探讨纺锤体检测机制变化在辐射致癌中的作用。方法：用噻氨酯哒唑药物破坏细胞的微管结构，导致纺锤体形成受阻，在光学显微镜下计数有丝分裂细胞。结果：本实验观察的辐射诱发癌变细胞系中有的染色体数目相对稳定，有的不稳定，其中表现为多倍体细胞比例增高。通过分析纺锤体检测点机制，发现染色体数目异常(多倍体)比例高的癌变细胞BERP35T4(40 ％)在噻氨酯哒唑药物作用后12～24 h，有丝分裂指数(MI)明显低于亲本BEP2D细胞和另一癌变细胞系BERP35T1，后二者细胞的多倍体率较低(5 ％)。结论：纺锤体检测点机制异常与α粒子照射诱发恶性转化细胞BERP35T4的染色体不稳定性相关。

关键词: α粒子辐射, 纺锤体检测点, 癌变

Abstract: Purpose: To analyze the spindle checkpoint function of BEP2D cells ant its malignant transformed cell lines induced by α-particles. Methods: Nocodazole, the inhibitor of spindle construction, was used to destroy the microtubule and initiate the spindle checkpoint mechanism to arrest the cells in the phase of mitosis, and mitotic index(MI) was counted under microscope. Results: BERP35T4 is a αparticles-induced transformed cell line with chromosome instability manifesting increased polyploids. After the treatment with nocodazole for 12～24 h, the mitotic index of BERP35T4 was significantly lower than that of the parental BEP2D cells and BERP35T1, another transformed cell line with relatively stable cytogenetic state. Conclusion: The spindle-checkpoint mechanism was deficient in BERP35T4 cells, and it could be related to the chromosome instability and the transformation of this cell line initiated by α particles exposure.

Key words: α-particles, spindle checkpoint, carcinogenesis

隋建丽, 孙敬芬, 周平坤, 吴德昌. α粒子诱发细胞癌变的纺锤体检测点功能分析[J]. 癌变·畸变·突变, 2003, 15(2): 65-66.

SUI Jian-li, SUN Jing-fen, ZHOU Ping-kun, et al.. THE SPINDLE CHECKPOINT MECHANISM OF BEP2D AND ITS MALIGNANT TRANSFORMED CELL LINES INDUCED BY α PARTICLES EXPOSURE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(2): 65-66.

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α粒子诱发细胞癌变的纺锤体检测点功能分析

THE SPINDLE CHECKPOINT MECHANISM OF BEP2D AND ITS MALIGNANT TRANSFORMED CELL LINES INDUCED BY α PARTICLES EXPOSURE

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